| Date | Home | Away | Result |
|---|---|---|---|
| 03/12/24 18:00 | Real Avila | Real Valladolid | 2-4a.e.t |
| 03/12/24 19:00 | Yeclano Deportivo | FC Elche | 0-1 |
| 03/12/24 20:00 | Salamanca CF UDS | Celta Vigo | 0-7 |
| 03/12/24 20:00 | UD Barbastro | Espanyol Barcelona | 2-0 |
| 03/12/24 20:00 | Real Saragossa | FC Granada | 6-7a.PSO |
| 03/12/24 20:15 | CE Europa | UD Las Palmas | 1-2 |
| 04/12/24 18:00 | Unionistas de Salamanca | Rayo Vallecano | 2-3 |
| 04/12/24 18:00 | Gimnastic de Tarragona | SD Huesca | 0-1 |
| 04/12/24 18:00 | UE Sant Andreu | Real Betis Sevilla | 1-3 |
| 04/12/24 19:00 | CF Ourense | Deportivo La Coruna | 1-0 |
| 04/12/24 19:00 | Cultural y Deportiva Leonesa | UD Almeria | 1-2 |
| 04/12/24 19:00 | FC Cadiz | CD Eldense | 0-1 |
| 04/12/24 19:30 | Racing Santander | Sporting Gijon | 1-0 |
| 04/12/24 20:00 | UD Logrones | FC Girona | 4-3a.PSO |
| 04/12/24 20:00 | CF Pontevedra | FC Villarreal | 1-0 |
| 04/12/24 20:00 | SD Ejea | FC Valencia | 1-3 |
| 04/12/24 20:00 | Zamora CF | CD Teneriffa | 1-3a.PSO |
| 04/12/24 20:00 | CD Estepona | CD Leganes | 6-7a.PSO |
| 05/12/24 18:00 | CP Cacereno | Atletico Madrid | 1-3 |
| 05/12/24 18:00 | AD Ceuta FC | CA Osasuna | 2-3 |
| 05/12/24 18:00 | Orihuela CF | FC Getafe | 0-3a.PSO |
| 05/12/24 19:00 | Andorra CF | FC Cartagena | 0-1 |
| 05/12/24 19:00 | Marbella FC | Burgos CF | 1-0 |
| 05/12/24 19:00 | SD Ponferradina | CD Castellon | 5-4a.PSO |
| 05/12/24 19:00 | Barakaldo CF | Racing de Ferrol | 1-2 |
| 05/12/24 20:00 | Ub Conquense | Real Sociedad | 0-1a.e.t |
| 05/12/24 20:00 | Olot | FC Sevilla | 1-3 |
| 05/12/24 20:00 | Deportiva Minera | Deportivo Alaves | 6-4a.PSO |