| Date | Home | Away | Result |
|---|---|---|---|
| 29/10/24 18:00 | SD Compostela | Deportivo Alaves | 0-1 |
| 29/10/24 18:00 | CD Villamuriel | Rayo Vallecano | 0-5 |
| 29/10/24 19:00 | CD Guijuelo | CF Ourense | 1-2 |
| 29/10/24 20:00 | CE L'Hospitalet | Real Saragossa | 1-5 |
| 29/10/24 20:00 | Real Jaen | FC Cadiz | 0-3 |
| 29/10/24 20:00 | UD Poblense | FC Villarreal | 1-6 |
| 29/10/24 20:00 | Astur CF | Real Valladolid | 1-4 |
| 29/10/24 20:00 | UD Lanzarote | Racing Santander | 3-4 |
| 30/10/24 17:30 | CE Europa | Albacete Balompie | 2-1 |
| 30/10/24 18:00 | CD Ibiza Islas Pitiusas | Gimnastic de Tarragona | 1-2 |
| 30/10/24 18:00 | Olot | Cordoba CF | 5-4a.PSO |
| 30/10/24 18:00 | Las Rozas CF | FC Sevilla | 0-3 |
| 30/10/24 18:00 | CF Lucena | CD Leganes | 1-2 |
| 30/10/24 18:30 | Bergantinos CF | Marbella FC | 1-2 |
| 30/10/24 18:30 | Beasain | FC Cartagena | 0-1 |
| 30/10/24 18:30 | UP Langreo | Orihuela CF | 1-2a.e.t |
| 30/10/24 18:30 | CD Mostoles | Burgos CF | 2-5 |
| 30/10/24 19:00 | UD Logrones | SD Eibar | 1-0a.e.t |
| 30/10/24 19:00 | Juventud Torremolinos CF | Zamora CF | 1-2 |
| 30/10/24 19:00 | CD Laredo | Yeclano Deportivo | 0-6 |
| 30/10/24 19:00 | Utebo | Unionistas de Salamanca | 0-4 |
| 30/10/24 19:00 | Club Lleida Esportiu | Barakaldo CF | 1-3 |
| 30/10/24 19:00 | CD Numancia | Sporting Gijon | 0-1 |
| 30/10/24 19:00 | UE Llagostera | SD Huesca | 0-2 |
| 30/10/24 19:00 | UM Escobedo | SD Ponferradina | 4-5a.PSO |
| 30/10/24 19:00 | Salamanca CF UDS | AD Alcorcon | 1-0 |
| 30/10/24 19:00 | UE Sant Andreu | CD Mirandes | 2-1 |
| 30/10/24 19:00 | CD Cortes | FC Granada | 0-2 |
| 30/10/24 19:30 | CD Coria | FC Elche | 0-3 |
| 30/10/24 19:30 | CP Cacereno | Gimnastica Segoviana | 2-1a.e.t |
| 30/10/24 19:30 | Ub Conquense | UD Ibiza Eivissa | 1-0 |
| 30/10/24 20:00 | San Sebastian de los Reyes | UD Almeria | 1-2 |
| 30/10/24 20:00 | CDA Aguilas FC | CD Castellon | 0-1 |
| 30/10/24 20:00 | UD San Pedro | Celta Vigo | 1-5 |
| 30/10/24 20:00 | CD Extremadura | FC Girona | 0-4 |
| 30/10/24 20:30 | CD Atletico Paso | CD Eldense | 0-1 |
| 31/10/24 18:00 | UD Barbastro | SD Amorebieta | 4-0 |
| 31/10/24 18:00 | San Tirso SD | Espanyol Barcelona | 0-4 |
| 31/10/24 18:00 | UE Vic | Atletico Madrid | 0-2 |
| 31/10/24 19:00 | CD Tudelano | Deportiva Minera | 0-5 |
| 31/10/24 19:00 | Real Avila | Real Oviedo | 3-0a.PSO |
| 31/10/24 19:30 | CD Don Benito | Andorra CF | 1-2 |
| 31/10/24 20:00 | CD Alfaro | CD Teneriffa | 0-2a.e.t |
| 31/10/24 20:00 | UD Llanera | Cultural y Deportiva Leonesa | 0-2a.e.t |
| 31/10/24 20:00 | Ontinena CF | UD Las Palmas | 0-7 |
| 31/10/24 20:00 | CD Gevora | Real Betis Sevilla | 1-6 |
| 31/10/24 20:00 | CD Cuarte | Racing de Ferrol | 1-3 |
| 31/10/24 20:00 | CD Estepona | FC Malaga | 3-2a.e.t |
| 05/11/24 18:00 | Chiclana | CA Osasuna | 0-5 |
| 06/11/24 18:00 | Deportivo Xerez | AD Ceuta FC | 0-1 |
| 14/11/24 17:00 | SD Ejea | CF Hercules | 1-0 |
| 19/11/24 19:00 | CF Pontevedra | UD Levante | 4-1 |
| 21/11/24 20:00 | Jove Espanol | Real Sociedad | 0-5 |
| 26/11/24 18:00 | Manises CF | FC Getafe | 0-3 |
| 26/11/24 18:00 | CP Parla Escuela | FC Valencia | 0-1 |