| Date | Home | Away | Result |
|---|---|---|---|
| 28/03/25 19:30 | Spezia Calcio | Brescia Calcio | 0-1 |
| 29/03/25 14:00 | Modena FC | US Catanzaro 1929 | 0-0 |
| 29/03/25 14:00 | Mantova 1911 | FC Südtirol | 1-0 |
| 29/03/25 14:00 | Sampdoria Genua | Frosinone Calcio | 0-0 |
| 29/03/25 14:00 | Cosenza Calcio | AC Pisa | 0-0 |
| 29/03/25 16:15 | US Cremonese | AS Cittadella | --- |
| 29/03/25 18:30 | Sassuolo Calcio | AC Reggiana 1919 | --- |
| 30/03/25 14:00 | FC Cesena | Juve Stabia | --- |
| 30/03/25 14:00 | Carrarese Calcio 1908 | SSC Bari | --- |
| 30/03/25 16:15 | US Salernitana | Palermo FC | --- |