| Date | Dead line | Home | Away | Result | Points rule |
|---|---|---|---|---|---|
| 24/09/21 17:30 | 24/09/21 17:15 | 1. FC Heidenheim 1846 | SV Darmstadt 98 | 2-1 | 2 - 4 points |
| 24/09/21 17:30 | 24/09/21 17:15 | Jahn Regensburg | Erzgebirge Aue | 3-2 | 2 - 4 points |
| 25/09/21 12:30 | 25/09/21 12:15 | Karlsruher SC | FC St. Pauli | 1-3 | 2 - 4 points |
| 25/09/21 12:30 | 25/09/21 12:15 | SC Paderborn 07 | Holstein Kiel | 1-2 | 2 - 4 points |
| 25/09/21 12:30 | 25/09/21 12:15 | FC Ingolstadt | Fortuna Düsseldorf | 1-2 | 2 - 4 points |
| 25/09/21 19:30 | 25/09/21 19:15 | FC Hansa Rostock | FC Schalke 04 | 0-2 | 2 - 4 points |
| 26/09/21 12:30 | 26/09/21 12:15 | Hannover 96 | SV Sandhausen | 1-2 | 2 - 4 points |
| 26/09/21 12:30 | 26/09/21 12:15 | Dynamo Dresden | Werder Bremen | 3-0 | 2 - 4 points |
| 26/09/21 12:30 | 26/09/21 12:15 | Hamburger SV | 1. FC Nürnberg | 2-2 | 2 - 4 points |